Gfp Cell Line

A549 cells gfp egfr is an a549 cell line in which the genomic egfr gene has been endogenously tagged with a green fluorescent protein gene gfp using compozr zinc finger nuclease technology.

Gfp cell line.

Cells are still been mainted in g418 at a lower. Green fluorescent protein gfp is a versatile biological marker for monitoring physiological processes visualizing protein localization and detecting transgenic expression in vivo gfp can be excited by the 488 nm laser line and is optimally detected at 510 nm. 1 vial x 2 x 106 cells vial product manual. Green fluorescent protein gfp has changed from a nearly unknown protein to a commonly used molecular imaging tool in biology genetics medicine and chemistry.

Product name description scc117. Gfp can be introduced into mammalian cells or whole organisms either by traditional plasmid transfection or viral infection. The follow expression cassette was integrated into cell line s genome. Histone h2b gfp expressing hela cell line.

Faseb bio advances doi. Non invasive imaging of gfp rfp labeled tumor stable cells has enabled the metastatic process be followed dynamically in real time in animals and screening of antitumor and antimetastatic drugs in vivo. The histone h2b gfp expressing hela cell line is a useful cell model for imaging and analysis of chromosome dynamics. Cell line contains a puromycin antibiotic selection marker.

Howell m c et al. 1 product result match criteria. At the moment stable cell line seems to have lost the expression of gfp i m so worried to what would have gone wrong and how it s possible. High level expression of gfp by ccl 243 gfp enables highly accurate quantitation of killing by flow cytometry.

10 1096 fba 2019 00081 gfp labeled h1975 cell line 2018. Integration resulted in endogenous expression of the fusion protein in which gfp is attached to the c terminus of egfr. Lung cancer cells survive epidermal growth factor receptor tyrosine kinase inhibitor exposure through upregulation of cholesterol synthesis. Gfp was expressed under the constitutive ef1a promoter.

The green fluorescent protein gfp is a protein composed of 238 amino acid residues 26 9kda that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Gfp reporter stable cell line hek293. Cellomics gfp rfp stable cancer cell lines express gfp rfp reporters under different promoters can be used for in vitro and in vivo cancer research.